Coding

Part:BBa_K2333009

Designed by: Ethan M Jones   Group: iGEM17_William_and_Mary   (2017-10-27)


mScarlet-I

mScarlet-I is the rapidly-maturing version of mScarlet, a monomeric red fluorescent protein designed by Bindels et al. in 2016 (see the full reference on the "Design" page). mScarlet-I is distinguished by a single amino acid substitution T74I from the original mScarlet protein. It possesses a 569nm absorbance and 594nm emission, and is notable for its high quantum yield (0.54) and fluorescence lifetime (3.1ns), as well as its rapid maturation time of ~36 minutes.

Improvement

An improved mScarlet-I is available in part BBa_K2680251. This part is compatible with 3G assembly and can be used with William and Mary 2018's protocols , to produce large numbers of multi-transcriptional unit circuits and circuit variants.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 512
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None